Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSKANHAKTVICGIINVTPDSFSDGGQFFALEQALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAALAAGADLVNDITGLMGDEKMPHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK 3TZF Chain:B ((20-278)) -----------VMGILNVTPDSFSDGGCHNNLDQALQHAQRMLSAGATLIDIGGESTRPG--EVSEQEELDRVVPVVEALAQRFDVWLSVDTSKAAVITESAHAGAHLINDIRSL---QEPGALEAAAKTGLPVCLMHMQGQPKNMQH---------SPYYDDLMTDINR---------FFQHHIERCVAAGIAKNKLLLDPGFGFGKNLAHNYQLLAHLSELHHFELPLLVGMSRKSMVGQLL-----NVPPQ------QRVIGSVACAVIAAMQGAQIIRVHDVKETVEAMCIVEATRSA------------

General information: TITO was launched using: RESULT: Template: 3TZF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1412 -149294 -105.73 -580.91 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -105.73 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3TZF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TZF-query.scw PDB file : Tito_Scwrl_3TZF.pdb: