Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKCADTFLLARDENQLLGYILSSPQS------------------DNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAVE-LDYKGIRLESP--------------------------DELLSYFEMNGFVDEEAT-LLYA-----TSQGYSMIWFNPFYLEEQ 2REE Chain:A ((11-208)) --NYYNLRHPKIEDLRDLIALETLCWSENLQVDNEEIYRRIFKIPQGQFILELEDKIVGAIYSQRIDNPQLLDNKTCTQVPLLHTESGVVVQLLAVNILPELQNQGLGDRLLEFMLQYCAQISGVEKVVAVTLCRNYPDYSPMPMAEYIHQKNESGLLVDPLLRFHQIHGAKIEKLLPGYRPKDWENQTCGVLVSYDIQH-----

General information: TITO was launched using: RESULT: Template: 2REE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 559 -25826 -46.20 -175.68 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -46.20 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2REE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2REE-query.scw PDB file : Tito_Scwrl_2REE.pdb: