Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDGAMQGSYATALLKQQQLPESFQFYFKQKQHLSNTQELHQLHELVRPRLKIAIVTETWPPEINGVALSLLQLCQGLQKQGHKILLVRPEQKAKCHDFLPE-QECLVMSQAIPKYPTLQFGW-PQYLKVSKAFE-KFVPDVVHIVTEGPLGLTAMQAAKAKDIPVSSGFHSPFQDFSRFFDLAFLVKPIQKYLCWFHNNTQVTCVPSKDTEEALRGFGITC-PLVVVGRGVDTTRFSPKHR-SENLRQQWGVDSDTRVMLYVGRLSPEKEVQLIVESYAAMQNIQQQKTKLVVVGDGP-----DLARLKALPEA----KNVIFTGSLCGHDLAVAYASADVFVFASQ-VETFGNVVLEAMASGLPVIAYDYACAHQYLIHGVNGWLSPLGHKNHFIQQIYQLPS-IQQLREMGIQACHKV-QQSGWQLPVQQLEQAFYQVVKELSADFT 4XSO Chain:B ((7-384)) -------------------------------------------------MKILFLDQS--GKPGGAELCLIDIAKPYRD-RALVGLFADGAFKTLLE-QHHIPVEVFTNQP---------SLGQLAPLVAKVVQTAHEYDLIYANTQKALV-VGAIASFIARRPLVYHLHDILSPEHFS------QTNLRVAVNLANRFASLVIANSQASQTAFIQAGGRAELTKVIYNGFDINLYKTSPSDISKLRQQLGV-ANNFVVGHFSRLSPWKGQHILIDALAQCP----PQVTAILVGDALFGEQDYVKELHQQITRLGLENRVKFLGFR--ADIPQLMAACDLVAHTSTAPEPFGRVIVEAMLCGKPVVAAKAGGAMELVEHGVNGFLTTPGESQELANIINTCIEDTQKTATIASNAQAIASQRFDVVTINQQIAETLSSL---------

General information: TITO was launched using: RESULT: Template: 4XSO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1923 -49323 -25.65 -142.14 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -25.65 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4XSO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XSO-query.scw PDB file : Tito_Scwrl_4XSO.pdb: