Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRPITAVIHRQALQNNLAVVRKAMP-NSKVFAVVKANAYGHGIERVYEAF-K-AADGFALLDLEEAKRIRALGWTGPILLLEGVFSPQDLFDCVQYQLSFTIHSEAQIEWVEQHPY-PAQFDVFLKMNSGMSRLGFKPQ-HYVQAWERLNNLANVAKITHMMHFSDADGDRFGQQGIDYQITAFEEIVKDLP---GERSVSNSAAILRYQDQLKSDYVRSGIMLYGSSPDYPTHSIADWGLQPTMSLRSEIISVQHLEPNESVGYGSNFVAEQPMTIGIVACGYADGYQRISPTGTPVLVDSVRTRTVGRVSMDMLAVDLTGIESAKVGSEVVLWGQSSTGVVLPIDDVAVSSGTVGYELMCAVTARVQFINQV 1BD0 Chain:B ((5-368)) -HRDTWAEVDLDAIYDNVENLRRLLPDDTHIMAVVKANAYGHGDVQVARTALEAGASRLAVAFLDEALALREKGIEAPILVL-GASRPADAALAAQQRIALTVFRSDWLEEASALYSGPFPIHFHLKMDTGMGRLGVKDEEETKRIVALIERHPHFVLEGLYTHFATADEVN--TDYFSYQYTRFLHMLEWLPSRPPLVHCANSAASLRFPD-RTFNMVRFGIAMYGLAPSPGIKPLLPYPLKEAFSLHSRLVHVKKLQPGEKVSYGATYTAQTEEWIGTIPIGYADGWLRRLQHF-HVLVDGQKAPIVGRICMDQCMIRLPG--PLPVGTKVTLIGRQGD-EVISIDDVARHLETINYEVPCTISYRVPRIF--

General information: TITO was launched using: RESULT: Template: 1BD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2009 -45706 -22.75 -128.39 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -22.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1BD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BD0-query.scw PDB file : Tito_Scwrl_1BD0.pdb: