Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDYTEKQVLQGKAVLFLGAGASYNCTDREKKRIGFTGNELLQKINEEFLGNTPNHLTLDFASTMAIQKAGRENFDLFIKE--LVCDFDP-TNEHKLLSEFKWKAIFTTNYDEAIEKAYRENKSALQKIEKIISDNDSLQKVVVNPDKLPIIKIHGCISRPNDTRTPLVISC-----SDYRRHQENRSSLYQYLKECLTSDLVIFYGYGLGDNNIVGILDDLEKEGVNRTRHIWLDPFMDEMYKDYWESKNLLCKVNNLYDFLS---EIKSKKDSNILSLNSFLKNDSVISKLIPSNDRPSNELETYLLKQLLYVEMNDEIKRESDNYNNDIFYRGNAHGFSWVAKNLDFQRAIERTLEVELFENFEISNQLFNFLIINGYAGSGKSVLLKRL-AWNGV--SKFNGNCFYLSEGATLNTKLIFELIKLIKEPIILFVDNILEHQTEILEIKNFSIKDCSKIMIVGAARSNEWNNSENILDKLNPNFFSLKDLNDFEVKNLVNKLKEFKAEGSLKELSDIDKYKFIKEKSNNQLLVTLLEATHYGQEFSEIIK-DEYEGIYDRSAKDLYLSICCFHRHGLE----LRAGMIKRLSGINFEQFKEKFLQPLELLVTSYYSYRVNDIVYTTRHSYIAQHVFKQAFLSEMDKAQQLIKIIRYLNIGFDTDSKALDFILKGRILAEEFNDKDLAHSIFNIAQDIGVNNAFILHQRAIFEINHQNPNYELALDYLKRINSSDGYYDMRTVEHTKANAYRKIALNSSSKQDKIKYRNLSLKLLNDNIRTSNRTSMPYHTKGLVLLDEIKDCDNENDLVDIIKDFENNLSAGFKKFPYDESLTVLEHDFSDEIDNSPRAIEKIKEALRKNSDNIYIVQRYAKFYIKKNDFLEARTCMLNFLRNDVNNRAINYLMAYSYIKENESLYFQNAIKYLKKCYSPRDSNYEPKFLHACLEYIYQSEEKALKIFTDLNQSNVSPKIKNKPTHMIRDSEGNERFFDAIIVTINDQFGFVKTSKYAENIYVHKSTISNEEDWEALRPGDPLKISIWFSFRGPRAKTAIIL 5C2V Chain:A ((1-781)) EPVMTGGPV-QGKALW----------TD-----YSGMSKEVQGPVSQILFTQSPRTAKGD----------PYQNYPHYIPEGSRIVLFDLNTKELKVLTND-----FATAFDPCT--YWDGKKFAFAGVHK---KGGGCQIWEMNIDGSGLRQMTD---LKGTCRSPIYYAAGSIEEGEGRIIWRDREGDWKEHGMVEKTGMIIFSGSPEGV--------------------------MDEFHNPY---------AYNLYRLDTQGGKIIQRITGHVLSGIEFPHLNTTIDQI------------TYNL---------------SSNFDPWLTPDGN---------------------------------ILFSSVQANGSRAGGEGRVMICVDNWDGAYPRPIYGNCDGEIGGTSGRSQAKITF----GDRKIVYVES---------PYMNWGV---GQLAAVS------WDAPF-------------------------NKTYEKLTGK------DGGLYRSPYPLPDDRMLVSYAERGDFGIYWFNFSKCAAGDKVYDDPNWNDHQPAPVYVKYKPRWINTFTAGKNFGVTVVTYQPFDQV---KVEGYPHSWGTWICFDTTLSDQP--VGPYPHQKAKNVSHGDIK-AVRIIQGYQCV-EPDSTRFRVGAGAHLLGGERS---------------SSNSGTAFQQRGIIGYQYVESDGSTVTSQL-----SDVPYYMQILDD------KGMSVQTALTWAYL--RPYHGRICSGCHYGSYRGRAFKNIHAKALYNWWYDDRSHYD---------SPFAFRYLKFDNDGNYKGVKHG-EDVVGTTSQPVEGLTLDKQRTVD----------FRRDIQPILDAKCAMCH-----DSNNPPNLGGGLELVSVDGIAAYSRAYNSLLEPQRGKDPNIGGK-------YVNPSAAINSLLVWRLYEAELSA---NAPREKIFPIEGR---------------------------LLHNKFLTQDERYAIVEWIDLG--AQWDNIPGPDFYPGYLV

General information: TITO was launched using: RESULT: Template: 5C2V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4812 156369 32.50 208.77 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 32.50 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.127

(partial model without unconserved sides chains): PDB file : Tito_5C2V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C2V-query.scw PDB file : Tito_Scwrl_5C2V.pdb: