Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKTLPLTDIHRHLDGNIRIQTILELGQQYNLDLPAYDIESLRPHVQVMDNQPDLLSFLSKLDWGVKVL-ASLDACKRIAFENMQDAAQQGLDYVELRFSPGYMGM------------AHQLPLEGVVEAVIAGVKEGSQAYGVKANLIGIMSRTFGQEACEKELNALLAH-KNDIKALDLAGDELGFP---GNLFIDHFKKARDAGWHITVHAGEAAGPESIWQAIEELGAERIGHGVKAVQDLKLLDYLAKHQIGIESCLTSNIQTNTVPSLAEHPLKTFLEHGVLATINTDDPAVEGIEIQHEYLTAAPLAGLSPEQIYTAQENGLKIAFLSEQEKDELRQKYQ 1ADD Chain:A ((1-344)) TPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIG-MDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEG-DVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPS-WSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFLPEEEKKELLERL-

General information: TITO was launched using: RESULT: Template: 1ADD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1955 15846 8.11 48.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 8.11 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_1ADD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ADD-query.scw PDB file : Tito_Scwrl_1ADD.pdb: