Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEHRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRAQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAHGR---PNALDPDEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSP----NTLLEILVPDFRGRMDIALRIMTECPPDVFNHNIETV-PRLYKAMRP---GSDYQHSLNLLKMFKEYCPDIP-TKCGLMVGIGETEEEVIALL----DDLRAH--DVDYVTIGQYL-QPSKQHAPIDRFVTPEEFERYAEHGRKLGFRNIWSAPMVRSSYFADRQYYGEPVPEVRRKVDPAKKIAVQAIEA 4WCX Chain:A ((95-299)) ---------------------------------------------------------------------------------FAPLYVSNYCVNNCRYCGYRHSNEQQRKKLTMEEVRREVEILEEMGHKRLAVEAGEDPVNCP--IDYIVDVIKTIYDTKLKNGSIRRVNVNIAAT--TV-ENYKKLKKVGIGTYVLFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEA--GIDDVGLGVLYGLYDYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNI----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4WCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 -28331 -34.22 -158.27 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -34.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_4WCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WCX-query.scw PDB file : Tito_Scwrl_4WCX.pdb: