Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYANGGDEMEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFILLILKEK-DGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVS---EGRLSLVYLTEQGRQFAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK 4AIK Chain:A ((4-138)) -------------------TLGSDLARLVRVWRALIDHRLKPLELTQTHWVTLYNINRLPPEQSQIQLAKAIGIEQPSLVRTLDQLEEKGLITRHTSANDRRAKRIKLTEQSSPIIEQVDGVISSTRKEILGGISSDEIAVLSGLIDKLEKNII--

General information: TITO was launched using: RESULT: Template: 4AIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 356 -19680 -55.28 -150.23 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -55.28 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.662

(partial model without unconserved sides chains): PDB file : Tito_4AIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AIK-query.scw PDB file : Tito_Scwrl_4AIK.pdb: