Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMTMSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYYKQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIFHGGRMTSRATLS-GEQPVSASAVAAKR--------PDAETPRAMSEEEIVETIQAFGKATRRAIESGFDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKPFIVGYRFSPEEYETPGIRF----EDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSILEVVHNTIQGKIPLVGVGGIRNREDVEQVLK-Y-AELAAIGQQMIVDPDWAEKLLENREDEFVTKPFEEAYKELYLPSPLYNFLKARYQ 5EPD Chain:A ((25-363)) -------TLFQPTSLG-AITLANRIVMAPLTRNRAGAGFVPGELTAGYYAQRA-SAGLIISEATQISQQGQGYQDTPGIYTQAQIDGWKKVTAAVHKKGGRIVLQLWHVGRISHVNLQPNGGAPVAPSAIRAEVKTFVNNGFVDVSEPRALELEELAGIVDDFRKAAANSIEAGFDGVEVHGANGYLLEQFAKDGANMRTDTYGGSVENRARLMLEVTAAVAQEIG-----PERTGIRISPVSPA-NGISCSDPQTQYDYIVDKLDALGIAYIHVVE-------------PFD-YGSLRRRF--SRTYIAN----DLELATSHLADGRADLIAFGRPFIANPDLVERLQSGAPLAEVNAAK----------------------

General information: TITO was launched using: RESULT: Template: 5EPD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1883 -3898 -2.07 -12.53 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -2.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_5EPD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EPD-query.scw PDB file : Tito_Scwrl_5EPD.pdb: