Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANMKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGAS---KVDIRLSDGTKVPGEIVGADTFSDIAVVKISSE-KVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPAN-GHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYR-NGKEETTSIKLNKSSGDLES 2Z9I Chain:A ((6-314)) --------------------------------------------------------------GSVEQVAAKVVPSVVMLETDL---------------------EEGSGIILSA---EGLILTNNHVIAAAAKPPKTTVTFSDGRTAPFTVVGADPTSDIAVVRVQGVSGL-TPISLGSSSDLRVGQPVLAIGSPLGL--EGTVTTGIVSALNRPVST------NTVLDAIQTDAAINPGNSGGALVNMNAQLVGVNSAIATL--QSGSIGLGFAIPVDQAKRIADELISTGKASHASLGVQVTNDK----------------TLGAKIVEVVAGGAAANAGVPKGVVVTKVDDRPINSADALVAAVRSKAPGATVALTFQDPSGGSRTVQVTLGKA------

General information: TITO was launched using: RESULT: Template: 2Z9I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1728 37195 21.52 133.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 21.52 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_2Z9I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z9I-query.scw PDB file : Tito_Scwrl_2Z9I.pdb: