Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYF 3WA4 Chain:A ((6-81)) WFFGKIPRAKAEEMLSKQRHDGAFLIRESE--SAPGDFSLSVKFGNDVQHFKVLRD-GAGKYFLW-----VVKFNSLNELVDYH

General information: TITO was launched using: RESULT: Template: 3WA4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 276 -49257 -178.47 -648.12 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -178.47 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_3WA4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WA4-query.scw PDB file : Tito_Scwrl_3WA4.pdb: