Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHY 1NZV Chain:B ((4-86)) WYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1NZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 307 -40659 -132.44 -489.86 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -132.44 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.670

(partial model without unconserved sides chains): PDB file : Tito_1NZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NZV-query.scw PDB file : Tito_Scwrl_1NZV.pdb: