Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELT--EDATLHISESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPT-DMFVEACKAVVRANEEYVPLYGIGGTLYLRPLLIGVGDI-IGVKPAEEYI--FTIFAMPVGNYFKGGLVPTNF-LIQDEYDRAAPNGT-GAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKIEEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDRFVEAGACGTAAVISP---IGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEGWIVKVD 1I1L Chain:C ((10-308)) -----------------------------WFNGEMVRWEDAKVHVM--SHALHYGTSVFEGIRCYDSHKGPV-VFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLT--------SAYIRPLIF-VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQEGIALDVNGY--ISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKE-LGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVG--------EGRCGPVTKRIQQAFFGLFTGETEDKWGWLDQVN

General information: TITO was launched using: RESULT: Template: 1I1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1529 -10731 -7.02 -37.26 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -7.02 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1I1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I1L-query.scw PDB file : Tito_Scwrl_1I1L.pdb: