Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGLGGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFKLLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTIVPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLRGAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTTAEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQAFLQALVKKLDRSTVDLIKHY 2CF4 Chain:A ((3-331)) --SMIEKLKKFTQIPGISGYEERIREEIIREIKDFA-DYKVDAIGNLIVELGE---GEERILFMAHMDEIGLLITGITDEGKLRFRKVGGIDDRLLYGRHVNVVTEKG-ILDGVIGATPPHLSLER-DKSVIPWYDLVIDIGAESKEEALEL-VKPLDFAVFKKHFSVLN-GKYVSTRGLDDRFGVVALIEAIKDLVDHELEGKVIFAFTVQEEVGLKGAKFLANHYYPQYAFAIDSFACCSPLTGDVKLGKGPVIRAVDNSAIYSRDLARKVWSIAEKNGIEIQIGVTGGGTDASAFQ--D-RSKTLALSVPIKYLHSEVETLHLNDLEKLVKLIEALAFE------------

General information: TITO was launched using: RESULT: Template: 2CF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1970 -192506 -97.72 -585.12 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -97.72 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_2CF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CF4-query.scw PDB file : Tito_Scwrl_2CF4.pdb: