Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIPQSLLEQSIQINIQQALQEDIGDGDITAMLTPEDEQATATIISREDMVLAGQPWVNALISAYDNTVQVTWLKQEGDRVAANEAFLKLAGSARSLLTVERPALNFIQTLSAVATKTAEYVQHLEGLNTKLLDTRKTLPGLRIAQKYAVTVGGGQNHRLGLFDAFLIKENHIMAAGGIAQAIAKAHQIAP-GKPVEVEVETWDELNQALEAGADIVMLDNFS--QQQMIDAVK--HVAGRCKLEASGNITIENLREVATTGVDYISMGVLTKDVKAVDLSMRFNA 2B7P Chain:B ((3-271)) -----------IRTFLERALKEDLGHGDLFERVLEKDFKATAFVRAKQEGVFSGEKYALELLEM--TGIECVQTIKDKERFKPKDALMEIRGDFSMLLKVERTLLNLLQHSSGIATLTSRFVEALNSHKVRLLDTRKTRPLLRIFEKYSVLNGGASNHRLGLDDALMLKDTHLRHVKDLKSFLTHARKNLPFTAKIEIECESFEEAKNAMNAGADIVMCDNLSVLETKEIAAYRDAHYPF-VLLEASGNISLESINAYAKSGVDAISVGALIHQATFIDMHMK---

General information: TITO was launched using: RESULT: Template: 2B7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1279 -76195 -59.57 -288.62 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -59.57 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2B7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B7P-query.scw PDB file : Tito_Scwrl_2B7P.pdb: