Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELPQETTTEQLLAEIEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVDGVTCLGFGRMAMGE-AAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAMMLLQANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRD-GGGVGDIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG 3P2O Chain:B ((3-284)) AMTLLDGKALSAKIKEELKEKNQFLKS-KGIESCLAVILVGDNPASQTYVKSKAKACEECGIKSLVYHLNENITQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLEGKDAVIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVGDVDFEEVSKKSSYITPVPGGVGPMTIAMLLENTVKSAKNRL-

General information: TITO was launched using: RESULT: Template: 3P2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1530 -103504 -67.65 -369.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -67.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_3P2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P2O-query.scw PDB file : Tito_Scwrl_3P2O.pdb: