Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLTVDEAAKEILAY--QQITFLTGAGVSTPSGVPDYRSL-SGIYHGMEQ-----PEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIH--LEIAHLAKEKNVWVVSQNIDGLHAKAKSP--QLVNFHGSLYQCHCRKCQQTV---DWKEYLHSD---KHQACGGQIRPDIVLYGEGFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEPYTFIQEDGAVVFKKIQELGADYD 5D7N Chain:F ((8-231)) --KLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLH-DKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVV-DFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINR-------------------------------

General information: TITO was launched using: RESULT: Template: 5D7N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 955 -12708 -13.31 -61.69 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain F : 0.74 3D Compatibility (PKB) : -13.31 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_5D7N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D7N-query.scw PDB file : Tito_Scwrl_5D7N.pdb: