Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILP-MSFIPQGTDLLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQ---DAIDGIGYPCVLKTT-RGGYDGKGQYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNG--QGDFTTFPVVENIHHNNILHETIAPAAID-QDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGE----QLLE------SYRLIENKPDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD 4E4T Chain:A ((30-409)) -----SPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALAELAGLCEAVSTEFENVPAASLDFLARTTFV-APAGRCVAVAQDRIAEKRFIEASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARL-GYDGKGQVRVSTAREARDAHAALGGVPCVLEKRLP-L-KYEVSALIARGADGRSAAFPLAQNVHHNGILALTIVPAPAADTARVEEAQQAAVRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQH-SPAAMLNILGDVWFPNGAAAGAVTPPWDTVAAMPAAHLHLYGKEEARVGRKMGHVNFTAEMRDDAVAAATA-----

General information: TITO was launched using: RESULT: Template: 4E4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2096 -58224 -27.78 -160.84 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -27.78 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_4E4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E4T-query.scw PDB file : Tito_Scwrl_4E4T.pdb: