Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGSHYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPKEMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLP--LKDEIIGANFSYMKYSSRYDQVLDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKI--STGVDGLGWGILRYENF-DVSIQPGKIGD------SYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKE-------IIDL---AVTAAENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNEEDF 5UI9 Chain:B ((5-344)) ELKGALIGCGFFAVNQMHAWKDVKGAGIAAICDRDPKRLKLVGDQFGI-ERRYGDAAALFADGGFDFVDIATTVQSHRALVEMAAAHKVPAICQKPFAKSLSDAKAMVRTCENADIPLMVHENFRWQTPIQAVKAVLESGAIGEPFWGRFSFRSGFDVF---S--GQPYLA--EGERFIIEDLGIHTLDIARFILGDVATLTARTKRVNPKIKGEDVATILLDHQNGATSIVDVSYATKLGTEPFPETLIDIDGTQGTIRLSQGYRL---EVTGPNGMTISDASPQLLSWASRPWHNIQESVLAIQQHWTDRLSSGG------ETSTSGADNLKTFALVEAAYESAANGRTVDIGAM-

General information: TITO was launched using: RESULT: Template: 5UI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1791 -86760 -48.44 -271.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -48.44 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_5UI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UI9-query.scw PDB file : Tito_Scwrl_5UI9.pdb: