Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYRLSMSLIANTTVVRGRFLDIQQTVSEPTDIPNQVRYLEDGVLISEHGKIKWFGAWEDAQQHLPAGVEVQHYPEQLIVPGFIDTHIHFPQTEMVG-AYGEQLLSWLNTYTFPTEIQFQDKTYASEIAQFFVQELLKHGTTTALVFCTVHPESVDALFEAAERVQMRLIAGKVLMDRNAPEALCDTPETAYSNTKALIEKWHGK-GRALYAITPRFAPTSTPEQLERAGQLKQEFPDVYVHTHLSENKDEIAWVKSLFPEQAGYLDVYQHYGLTGK-RSVFAHCVHLEDEEWQCMHDTQSAIAFCPTSNLFLGSGLFPLKKTWEKQVKVGLGTDIGAGTSFSLLQTVNEAYKVQQLQGDK-------LSAYEALYHATLGGAKALDLQDQLGNFNIGKEADFVVLNLKPTALQELRQTKSKSVEDSLFALFTLGDDRNIEATYIYGNRAYQKETAK 2I9U Chain:A ((9-427)) ------------NLKIFKGNLIFTKT--------SDKFTIMKDSYIVVIDGKIASVSSNLPDKYK---GNPIIDFRNNIIIPGMNDLHAHASQYKNLGIGMDKELLPWLNNYTFPEEAKFLNVDYAKKTYGRLIKDLIKNGTTRVALFATLHKDSTIELFNMLIKSGIGAYVGKVNMDYNCPDYLTENYITSLNDTEEIILKYKDKSNIVKPIITPRFVPSCSNELMDGLGKLSYKY-RLPVQSHLSENLDEIAVVKSLHKKSNFYGEVYDKFGLFGNTPTLMAHCIHSSKEEINLIKRNNVTIVHCPTSNFNLGSGMMPVRKYLNLGINVVLGSDISAGHTCSLFKVIAYAIQNSKIKWQESGKKDMFLSTSEAFYMATKKGGSFFG---KVGSFEEGYDFDALVINDSNLYPED------YDLTERLERFIYLGDDRNIMKRYVCGNEIF------

General information: TITO was launched using: RESULT: Template: 2I9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2525 -39812 -15.77 -97.34 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -15.77 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_2I9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9U-query.scw PDB file : Tito_Scwrl_2I9U.pdb: