Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKVEVPMSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDGEIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYARQDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANYFLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVKGKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLVVTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK 5T3O Chain:C ((1-307)) ----------------PLLIFSGQSNRPLAQAIAEALGLPLGKSTTLRFANDNLFVRYEESLREGDVFIVQSFVPPVQDHLMELLMMVDAAKGASAARVTAVIPYFSYARSDKKDAPRISITARLIADLLQTAGADRVLTMTLHSPQVHGFFKIPVDHLSAEPVIANYF-ATRVDLENAVVVAPDAGDLKRASALARRLGLPLAFIDKERVSDTEVRVRMLVGEVEGKTALIVDDEISTAGSLVEAVEALMQAGAKEVYAAATHGVYVGPALDRIAKSPVKEVAATDTCPPKEG---PKLRTLTVAPLFAEAIWRIHRGESVSSLFT----

General information: TITO was launched using: RESULT: Template: 5T3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1706 -258259 -151.38 -841.23 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -151.38 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_5T3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T3O-query.scw PDB file : Tito_Scwrl_5T3O.pdb: