Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFSPLCWIVPSIYVIMKEGFKIGKGVFMLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFASRYQNIQLFDQLEVFFKS-SFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEWFDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLAD---KQVLGADFNYAKYSSKMPDLLAGQTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQL--DNSIDLNGDGILFYPD-YQVHIKAGKNIT------SNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQ--------VQL---PIQQAPHTMTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK 5UHW Chain:A ((4-340)) ---------------------------TELKGALIGCGFFAVNQMHAWKDVKGAGIAAICDRDPKRLKLVGDQFGIERRYGDAAALFADGGFDFVDIATTVQSHRALVEMAAAHKVPAICQKPFAKSLSDAKAMVRTCENADIPLMVHENFRWQTPIQAVKAVLESGAIGEPFWGRFSFRSGFDV---FS--GQPYLA--EGERFIIEDLGIHTLDIARFILGDVATLTARTKRVNPKIKGEDVATILLDHQNGATSIVDVSYATKLGTEPFPETLIDIDGTQGTIRLSQGYRL---EVTGPNGMTISDASPQLLSWASRPWHNIQESVLAIQQHWTDRLSSGG--ETSTSGADNLKTFALVEAAYESAANGRTVD-

General information: TITO was launched using: RESULT: Template: 5UHW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1674 -57433 -34.31 -183.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -34.31 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_5UHW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UHW-query.scw PDB file : Tito_Scwrl_5UHW.pdb: