Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYATDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLTMHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIGDYDYK--VLMHY 4M0K Chain:D ((30-198)) -TLKQAIRTVPDFPEPGIQFKDITPVLGHPELLRLAIEALLEPFQEQEITKVVGIESRGFILGGMLAHHLDAGFVPVRKKGKLPYQTLAESYQLEYGTDTIEMHIDAIEPGDRVLIHDDVIATGGTAEATIRLVERAGGEVVGCAFLIELTGLQGRKRLP-AHVPVHTVLQ-

General information: TITO was launched using: RESULT: Template: 4M0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 849 -135260 -159.32 -809.94 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -159.32 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4M0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M0K-query.scw PDB file : Tito_Scwrl_4M0K.pdb: