Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKL-CDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISVSQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQGKDLQQVLADLKTLLTDNGFVVDYVEARQPNLLAASQFDRDIVLFVAAKLGGTRLIDNLQVAFTPQ 3IOD Chain:A ((32-286)) -------------------GRRVM-LVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNPMQF-----LDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLR-TTVQPGPLAAELEGGPRPTHFAGVLTVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAA-VALSAALTAAAHAATAGAQAALDAARAVLDAAPGVAVDYLELRDIG-LG----NGSGRLLVAARLGTTRLLDNIAI-----

General information: TITO was launched using: RESULT: Template: 3IOD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1268 -64175 -50.61 -261.94 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -50.61 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_3IOD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IOD-query.scw PDB file : Tito_Scwrl_3IOD.pdb: