Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLRIEKNNGIATVYLNRPDKRNAMSFALLKELVTTAKAIQKDRDIRCVILTGEAQVFSAGIDLSDLNNPKNSAFAIWELVKPGQSLFQKAFLIWQNLPVPVIAALEGYCFGAGMQLALAADIRIAHPDTKMSIMESRWGLVPDMGLTRSLKGLIGVDLAKELTLTARVLDGNYAKDIGLVTHLSENPLEKANAIASEMLQRSPDALTAAKRVLDAMEHQPEKSLRLEKIWQLKLLLGKNSKLARKKDKHPEVKFLPRQYR 3PE8 Chain:A ((10-203)) --VLLVDTTDRVRTLTLNRPQSRNALSAELRSTFFRALSDAQNDDDVDVVIVTGADPVFCAGLDLKEL--------------SPK----------WPDMTKPVIGAINGAAVTGGLELALYCDILIASENAKFADTHARVGLMPTWGLSVRLPQKVGVGLARRMSLTGDYLSAQDALRAGLVTEV----------VAHD------DLLTAARRVAASIVGNNQKAVR-----------------------------------

General information: TITO was launched using: RESULT: Template: 3PE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1032 -133297 -129.16 -720.52 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -129.16 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_3PE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PE8-query.scw PDB file : Tito_Scwrl_3PE8.pdb: