Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MALISMRQLLDHAAEHNYGVPAFNVNNLEQMRAIMLAADATNSPVIVQASAGARKYA--GAPFLRHLILAAIEEWPHIPVVMHQDHG-----------TSPDVCQRSIQLGFSSVMMDGSLGADGKTPTTYDYNVDVTRQVVAMAHACGVSVEGEIGCLGSLETGMAGEEDGVGAEGVLDHSQLLTSAEEAKQFVADTNVDALAIAVGTSHGAYKFTRPPTGDILAIDRIKEIHVALPNTH---LVMHGSSSVPQEWLKVINEFGGNIGDTYGVPVEQLVEAIKHGVRKINIDTDLRLASTGAIRRFMAENPA-EFDPRKYFAKTVDSMKQICIDRYEAFGTAGNADKIRPISLEKMVDRYK 1GVF Chain:A ((2-285)) SIIS---TKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPVILAGTPGTFK--HIALEEIYALCSAYSTT-YNMPLALHLDHHESLDDIRRKVHA-----------GVRSAMIDGS-------HFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGS---------------------AFLTDPQEAKRFVELTGVDSLAVAIGTAHG-LYSKTPK----IDFQRLAEIREVV----DVPLVLHGAS---------------------DVPDEFVRRTIELGVTKVNVATELKIAFAGAVKAWFAENP-QGNDPRYYMRVGMDAMKEVVRNKINVCGSANRIS---------------

General information: TITO was launched using: RESULT: Template: 1GVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1334 35242 26.42 138.75 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 26.42 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_1GVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVF-query.scw PDB file : Tito_Scwrl_1GVF.pdb: