Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMTMSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYYKQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIFHGGRMTSRATLS-GEQPVSASAVAAKR--------------PDAETPRAMSEEEIVETIQAFGKATRRAIESGFDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKPFIVGYRFSPEEYETPGIRFED----TIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSILEVVHNTIQGKIPLVGVGGIRNREDVEQVLK-Y-AELAAIGQQMIVDPDWAEKLLENREDEFVTKPFEEAYKELYLPSPLYNFLKARYQ 1GWJ Chain:A ((4-354)) -TSFSNPGLFTPLQLG-SLSLPNRVIMAPLTRSRT-PDSVPGRLQQIYYGQRA-SAGLIISEATNISPTARGYVYTPGIWTDAQEAGWKGVVEAVHAKGGRIALQLWHVGRVSHELVQPDGQQPVAPSALKAEGAECFVEFEDGTAGLHPTSTPRALETDGIPGIVEDYRQAAQRAKRAGFDMVEVHAANACLPNQFLATGTNRRTDQYGGSIENRARFPLEVVDAVAEVFG-----PERVGIRLTPFLEL-FGLTDDEPEAMAFYLAGELDRRGLAYLHFNEPDWIGGDIT---YPEGFREQMRQRF--KGGLIYCGNY-DAGRAQARLDDNTADAVAFGRPFIANPDLPERFRLGAALNEPDPST----------------------

General information: TITO was launched using: RESULT: Template: 1GWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2026 2039 1.01 6.18 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 1.01 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_1GWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GWJ-query.scw PDB file : Tito_Scwrl_1GWJ.pdb: