Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRY-LNRVNKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVEGISKPEI----YAKLLPYTAEWTEVLIKGIAPKKK 3ANP Chain:A ((2-196)) --VREYQKKRRRERIFRAAMELFRN-RGFQETTATEIAKAAHVSRGTFFNYYPYKEAVLLDYGSQLLAGLREEVRRLLAQGREPVEVLRHLFRVLAEGTARE--KDLLLPMFYELLNPDPVRAR-A-AFEALPLGDLIAEILKPLREQGVLRQDFSLERMGRTLADLYFLSALRWAAYTPGRDLAEELEKNLRLLLEGMLVR--

General information: TITO was launched using: RESULT: Template: 3ANP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 703 -63755 -90.69 -335.55 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -90.69 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_3ANP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANP-query.scw PDB file : Tito_Scwrl_3ANP.pdb: