Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGLIADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVLCLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAFTKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESMVRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEIDYINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE 1KS9 Chain:A ((1-291)) MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLV----ETDGSIFNESLTANDPDFLAT----SDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGTIEELQN--IQQPLLMGTTTHAARRDGN-VIIHVANGITHIGPARQQDGD-YSYLADILQTVLPDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHH---PQEIMQICEEVAAVIEREGHHTSAEDLRDYVMQVIDATA--ENISSMLQDIRAL-RHTEIDYINGFLLRRARAHGIAVPENTRLFEMVKRKESE----

General information: TITO was launched using: RESULT: Template: 1KS9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1563 -216368 -138.43 -743.53 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -138.43 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1KS9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KS9-query.scw PDB file : Tito_Scwrl_1KS9.pdb: