Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRL--------AEFLKNHQDCLQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREISSQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLVETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTGQDAERLAPYFNGILVGTALMQAENVVQRIKELQIDKG 1JCM Chain:P ((1-254)) -MQCVLAKIVADKAIWVEAR--KQQQPLASFQNEVQPSTRHFYDALQ--GARTAFILECKKASPSKGVIRDDFDPARIAAIYK-HYASAISVLTDEKYFQGSFNFLPIVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSNEEEQERAIALGAKVVGINNRDLCDLSIDLNRTRELAPKLGHNVTVISESGINTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLL---

General information: TITO was launched using: RESULT: Template: 1JCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1349 -2714 -2.01 -11.03 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain P : 0.75 3D Compatibility (PKB) : -2.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_1JCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JCM-query.scw PDB file : Tito_Scwrl_1JCM.pdb: