Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANMKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQ--------NS-VFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSE-KVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPAN-GHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES 2ZLE Chain:H ((2723-3032)) ------------------------------------------------------------QMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFG-----GQQQKFMALGSGVIIDAD--KGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRSDIALIQIQNPKNL-TAIKMADSDALRVGDYTVAIGNPFGL--GETVTSGIVSALGR-------------ENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPD--GGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSEL---AKAMKVD--AQRGAFVSQVLPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQ--------

General information: TITO was launched using: RESULT: Template: 2ZLE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1564 43366 27.73 145.04 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain H : 0.74 3D Compatibility (PKB) : 27.73 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_2ZLE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZLE-query.scw PDB file : Tito_Scwrl_2ZLE.pdb: