Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKKFLFQIHWFLGISAGLILSIMGVTGAIYSYDQQILKWVNTDSYVVQAQSSPKLTPAQLYQHFTTIQPEIKINSITIAKDATASSVVNIEKEGERRGYNMMVNPYTAQVLPEVQGRKLLLLIQQIHRNLTAGEFGKQITGACALMLIYFVLSGLYLRWPKKHSARQWLAVKPKLKGRNFIWDLHAVVGTWVIVFYLLFACTGLYWSYDWWRSGMFKVLGVEQPKMQGHGGSGRNKDQLPKIQLDNAQLITALNQTWSGFNNQIGRDYSTLTVNLPKKDDGKIELSFVDATPQHERARNQAVYNYKTANIEKMELYEDKKLNQKIMSSMLPVHRGSFFGPVYQFVAMLASLAMPLFFVTGWMLYLKRRKQKKLTQAARQSLAGHYIDQNAKPWLITYATQTGVAEQLAWRTATSLQEAHQPVQVKSVQQLTEADLQQHEQILFVISTYGTGEAPDLASNFAKKLLKTNLRLQHVKYAVLALGSKEYPDTYCSFGHTVDDWLKNNGAKALFDIIEVDNANPADIQNWNQALVKATKLDLHAVNIEKVFDNWTLQQRDLLNPNSLGQPAYNIELTANH--EAIWQAGDIAEIQPGNSPERINKFLQHHHILKNAV--VDSL-------------------Q-VSI---------------E-------------------KAL------------------WNKDLTG-EIEPFANLDHLLEQLPTLPTREYSIASIPSQ--QVLRLVVRQQYDESDDLDLDLGLGLGLGLGLGLGSGWLTQHTEINQNVALRIRTNESFHLIDD-NRPIICIGNGTGIAGLMSLLHTRTRH------NYTENWLIFGERQRAHDFFYASTIEAWQTMGMLKRLDLAFSRDQE-QRVYVQDIIQQN-AAELINWI-ERGAVLYVCGSIDGMASGVDQALIHILGEE----------QVDELRQQGRYRRDVY 1F20 Chain:A ((28-433)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTR--------DGEGPVHHGVCSSWLNR-IQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAAD-VLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIF

General information: TITO was launched using: RESULT: Template: 1F20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1443 -27575 -19.11 -89.82 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -19.11 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_1F20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F20-query.scw PDB file : Tito_Scwrl_1F20.pdb: