Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLPQQYRAKIIGYPSFGDTPESLEVKSFEDLTNYVVNQI----HDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLE--PFNVQ----DWR-----------------------EAYRQAFL---KYP-DWFIT--------TNANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 5EGN Chain:H ((20-259)) VPIVFLHPFSTNGGIWYFQTFPFAQTNHVIVIDHRGHGRSDKPATGYSIMEHADDVVAVLDALKVDRAVFVGNSIGGMIAMQLNLDHPQRVIGNLILSSGTGLGEGMPPEAGAAFQNDYIGAFGGLLEGAVSARSKRERPEILAVMKAHFSVPSNFPKHVFDAATADPNGVFAWNIKDRLSSIQAPTLVVAGEEDLVTTVANNQLLADNIPGAELRVINDVGHFYQLERPSEFNELLRGF-----

General information: TITO was launched using: RESULT: Template: 5EGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 933 -40734 -43.66 -208.89 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain H : 0.68 3D Compatibility (PKB) : -43.66 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_5EGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGN-query.scw PDB file : Tito_Scwrl_5EGN.pdb: