Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYCRNMNRSKAEQLLRSEDKEGGFMVRDS-SQPGLYTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGSIPEIIEYH 3N8M Chain:A ((8-83)) WFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSV--KFGNDVQ------HFKVLRDGAGKYFLWVV-KFNSLNELVDYH

General information: TITO was launched using: RESULT: Template: 3N8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 259 -10834 -41.83 -144.45 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -41.83 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_3N8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N8M-query.scw PDB file : Tito_Scwrl_3N8M.pdb: