Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRELPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGGTLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSHLLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGSTIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD 1K82 Chain:A ((1-268)) -PELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYR-LSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAW--LWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK--------PGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK--

General information: TITO was launched using: RESULT: Template: 1K82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1397 -137503 -98.43 -528.86 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -98.43 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_1K82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K82-query.scw PDB file : Tito_Scwrl_1K82.pdb: