Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYATDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLTMHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIGDYDYK--VLMHY 4LZA Chain:B ((27-195)) -EIKMMIREIPDFPKKGIKFKDITPVLKDAKAFNYSIEMLAKALEGRKFDLIAAPEARGFLFGAPLAYRLGVGFVPVRKPGKLPAETLSYEYELEYGTDSLEIHKDAVLEGQRVVIVDDLLATGGTIYASAKLVESLGGIVDSIIFLTELTFLDGRKKLD--GYDIISLIKF

General information: TITO was launched using: RESULT: Template: 4LZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 823 -131493 -159.77 -787.38 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -159.77 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_4LZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LZA-query.scw PDB file : Tito_Scwrl_4LZA.pdb: