Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGPRPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQ-LDIPVLGVILPGASAAIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSVTKKVVYETLRPLVG-KVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLLNYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL 3OUT Chain:C ((5-258)) LDNRPIGVFDSGIGGLTIVKNLMSILPNEDIIYFGDIARIPYGTKSRATIQKFAAQTAKFLIDQEVKAIIIACNTISAIAKDIVQEIAKAIPVIDVITAGVSL-VD--NLNTVGVIATPATINSNAYALQIHKKNPNIEVYSNPCGLFVSMIEEGFVSGHIVELVAKEYLSYFHDKNIQALILGCTHYPIIKESIAKIL--DVKLIDPSLQASKMLYSLLFENKLLNTT-KSNPEYRFYVTDIPLKFRSVGEMFLQTEMQ------

General information: TITO was launched using: RESULT: Template: 3OUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1503 -113748 -75.68 -451.38 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -75.68 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_3OUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OUT-query.scw PDB file : Tito_Scwrl_3OUT.pdb: