Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTISHIDPEYQANTIEPSVQQDWENRKVFKVADTVEGKHR-YILSMFPYPSGKLHMGHVRNYTIGDVISRFYRLKGETVLQPMGWDAFGLPAENAAIAHKVAPAKWTFENIAYMRDQLKKLGLSVDWDREFATCTPEYYHWEQWLFVQLYKKGLIYRKLSTVNWDPVDQTVLANEQVENGRGWR-SGALVEKRDIPMYYFRITDYAQELLDDLDTLQDGWPQQVLTMQRNWIGRSTGMEITFPSANTEIYADGLTVYTTRADTLMGVTYVAVAAEHPLALK--AAENNPELAAFIEECRIGSVAEADLATAEKKGMATGLFVKHPVTGEELPVWIANYVLMSYGSGAVMAVPAHDERDFEFANKFNLPIKQVIDAKGADDADYSATEWQEWYGSKEGKLVNSGEFDGLEFQAAFDAFLAKLEPQGLANSKVQFRLRDWGVSRQRYWGCPIPMINCDTCGQVTVPEDQLPVVLPTDVVPDG----SGNPLNKMPEFYETKCPCCGGDARRETDTLDTFVESSWYYARYASPDFTGGIVKPEAAKNWLPVNQYIGGVEHAILHLLYARFFHKLMHDEGVVQGNEPFTNLLTQGMVLADTFYREAENGKKTWFNPANIELERDEKGRIISAKYSG---------DGQEVIIGGQEKMSKSKNNGIDPQAIIDQYGADTARVFMMFAAPPDQSLEWSDAGVEGANRFLKRVWRLVASFLEKGNSATA-IDKANLSKDAQDLRRKTHETIQKVSDDIERRHAFNTAIAALMELLNASNKFEAKDDNDVAVEREAITTLLTLLAPFAPHLSQTLLAQFGTD-LTEATFPEVDASALTRNTQTIVVQVNGKLRGKLEVSVDISKDELLAQAKALPEVQQFLTGPT-KKEIVVPNKLVNLVV 2BYT Chain:A ((1-876)) ME------KYNPHAIEAKWQRFWEEKGFMKAKDLPGGRGKQYVLVMFPYPSGDLHMGHLKNYTMGDVLARFRRMQGYEVLHPMGWDAFGLPAENAALKFGVHPKDWTYANIRQAKESLRLMGILYDWDREVTTCEPEYYRWNQWIFLKMWEKGLAYRAKGLVNWCPKCQTVLANEQVVEGRCWRHEDTPVEKRELEQWYLRITAYAERLLKDLEGL--NWPEKVKAMQRAWIGRSEGAEILFPVEGKE---VRIPVFTTRPDTLFGATFLVLAPEHPLTLELAAPEKREEVLAYVEAAKRKTEIERQAEGREKTGVFLGAYALNPATGERIPIWTADYVLFGYGTGAIMAVPAHDQRDYEFARKFGLPIKKVIERPGEPLPEPLERAYEE-----PGIMVNSGPFDGTESEEGKRKVIAWLEEKGLGKGRVTYRLRDWLISRQRYWGTPIPMVHCEACGVVPVPEEELPVLLPDLKDVEDIRPKGKSPLEAHPEFYETTCPKCGGPAKRDTDTMDTFFDSSWYYLRYTDPHNDRLPFDPEKANAWMPVDQYIGGVEHAVLHLLYSRFFTKFLHDLGMVKVEEPFQGLFTQGMVLAWTDFGPVEVEGSVVRLPEPTRIRLEIPESALSLEDVRKMGAELRPHEDGTLHLWKPAVMSKSKGNGVMVGPFVKEQGADIARITILFAAPPENEMVWTEEGVQGAWRFLNRIYRRVAEDREALLETSGVFQAEALEGKDRELYGKLHETLKKVTEDLEALR-FNTAIAALMEFLNALYEYRKDRPVT-PVYRTAIRYYLQMLFPFAPHLAEELWHWFWPDSLFEAGWPELDEKALEKDVVEVAVQVNGRVRGTIHIPKDAPLEVARAEALKVRNVRAHLEGKEVVKEIYVPGKILNLVV

General information: TITO was launched using: RESULT: Template: 2BYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4588 26324 5.74 30.75 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 5.74 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_2BYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BYT-query.scw PDB file : Tito_Scwrl_2BYT.pdb: