Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMSQIVDIRAREILDSRGNPTIEADVILESGVVGRACAPSGASTGSREALELRDGDKSRYLGKGVRTAVQNVNSSIHELLV--GQSVFEQKALDEKMIAFDGTENKSKLGANATLAVSLAAAHAAAAEQKLPLFQYIANLRGQTTLTMPVPMMNILNGGAHADNTVDIQEFMIEPVGFTSFAEALRAGAEVFHSLKSVLK-KQGLN-TAVGDEGGFAPNLRSNEEAITVILQAIEQTGYKAGSDIMLALDCASSEFYKNGQYILE----GEGNKSFTSNQFADYLAGLVKQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDLFVTNPKILQRGIDEKVGNSILIKYNQIGTLTETLDAIYLAKANGYTTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEELTKAVYRGKAEFKGLK 4ZA0 Chain:A ((3-415)) -----IEKIWAREILDSRGNPTVEVDLYTAKGLF-RAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYT--EKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVG--IQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEE----------------

General information: TITO was launched using: RESULT: Template: 4ZA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2622 47627 18.16 117.60 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 18.16 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4ZA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZA0-query.scw PDB file : Tito_Scwrl_4ZA0.pdb: