Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLIQPELFFSALSPAEQRIQQALEDIRQGKPVLVMDDFDRENEADLIVAAETLTVETMVRMIHDGSGIVCLCLTEELADHLELPPMVSQNSSQFHTAFTVTIEAAQGVTTGVSAKDRVTTIKTAIKDGAVASDLNRPGHVFPLRARNGGVLTRRGHTEGTIDLARLAGLKPAGVLCELTN--PDGTMASGIQVLAYAQTHHLTVITIEELVQYRQQHGI 3MIO Chain:B ((2-206)) ---------------TRLDSVERAVADIAAGKAVIVIDDEDRENEGDLIFAAEKATPEMVAFMVRYTSGYLCVPLDGAICDRLGLLPM------------TVTVDARNGIGTGISASDRATTMRLLADPTSVADDFTRPGHVVPLRAKDGGVLRRPGHTEAAVDLARMAGLQPAGAICEIVSQKDEGSMAHTDELRVFADEHGLALITIADLIEWRRKHE-

General information: TITO was launched using: RESULT: Template: 3MIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1097 -92649 -84.46 -485.07 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -84.46 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.607

(partial model without unconserved sides chains): PDB file : Tito_3MIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MIO-query.scw PDB file : Tito_Scwrl_3MIO.pdb: