Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEKFSVEKVLSVHRWTPTLFSFTMTRPSHFKFTAGQFARIGLKVGDELIVRAYSVVSSPFDETLEFFSIVVPDGAFTSNLQHLKVGDELYLE-KIPYGYLTLARYQQPLPHDLWLLATGTGLAPFLSMLQDFETWSNYQKINLVYSVRTAAELAYVDRIQEIAETFGE----GHLGFKFIPIITRDPSAPLHERLPVLIENGE-LEKVAGLELSPASSHVMLCGNPQMVDDTKEALKRCGLTMN-RRGEGNIAVENYW 4FK8 Chain:B ((17-269)) ---SKFDTATVLSVHHWTDTLFSFTCTRDQALRFNNGEFTMVGLEVDGKPLTRAYSIVSPNYEEHLEFFSIKVQNGPLTSRLQHLKVGDPVLIGKKP-TGTLVAD--NLLPGKTLWMLSTGTGLAPFMSIIRDPDIYERFDKVVLTHTCRLKGELAYMDYIKHDLPGHEYLGDVIREKLVYYPTV----------RITDLIASGKLFTDLDMPPFSPEQDRVMLCGSTAMLKDTTELLKKAGLVEGKNSAPGHYVIERAF

General information: TITO was launched using: RESULT: Template: 4FK8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1241 -79992 -64.46 -337.52 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -64.46 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4FK8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FK8-query.scw PDB file : Tito_Scwrl_4FK8.pdb: