Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRY-LNRVNKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVEGISKPEI----YAKLLPYTAEWTEVLIKGIAPKKK 3ANP Chain:B ((10-196)) ----------RRERIFRAAMELFRN-RGFQETTATEIAKAAHVSRGTFFNYYPYKEAVLLDYGSQLLAGLREEVRRLLAQGREPVEVLRHLFRVLAEGTARE--KDLLLPMFYELLNPDPVRAR-A-AFEALPLGDLIAEILKPLREQGVLRQDFSLERMGRTLADLYFLSALRWAAYTPGRDLAEELEKNLRLLLEGMLVR--

General information: TITO was launched using: RESULT: Template: 3ANP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 690 -62080 -89.97 -341.10 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -89.97 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_3ANP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANP-query.scw PDB file : Tito_Scwrl_3ANP.pdb: