Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTDTALIMALPNESKGLFEQAGIEVHYSGIGKINAAFKAFEVIQKT-----GCKTLINLGTAGSSSFNRHDLVEIKTFVQRDMDVSPLGFEVGVTPLDDHLAAEIHLQTHFADLPKGICGTGDSFETGQPKV--ACDVVDMEGYALAKVCHKLGVRLISVKYITDGADDTAHLDWEENLLLGAQKLLALYQNHF 4PR3 Chain:B ((30-221)) -GKRLLYVMAADAEYGRHLA-KLFTPLMIGVGPVEAAVNLASALAHLKLAGDMPDLVISLGSAGSAKLPQAEVYQVSSVSYRDMDASPIGFEKGVTPFLDLPETVELPF-RVAGIDTASLSTGGNIVSGKAYERIEADMVDMETYACLRACQAVGVPLLGLRGISDGASEL---------HVIDEKLAGAVARV-

General information: TITO was launched using: RESULT: Template: 4PR3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 928 -20649 -22.25 -117.99 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -22.25 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_4PR3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PR3-query.scw PDB file : Tito_Scwrl_4PR3.pdb: