Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQKKSTQKRNQLLAAALDVFSLYGFSGASLDEIAQLADMHKSNIFYYYENKESLYVEVLTTVLQKWLAPLQTL-EAELEPAEALSHYLIQKIELSRSQPKASRLFALEIIQGAPHILPILKGPLKKLFKRKAKVIQTWQEQGKISPDIDPELLILNIWGLTQNYADFATQMEMVTGKTLRNRSMFQRSIEHTVHMMLYGVLPR 3ANP Chain:B ((10-195)) --------RRERIFRAAMELFRNRGFQETTATEIAKAAHVSRGTFFNYYPYKEAVLLDYGSQLLAGLREEVRRLLAQGREPVEVLRHLFRVLAEGTAREKDLLLPMFYELLNPDPVRARA-AFEALPLGDLIAEILKPLREQGVLRQDFSLERMGRTLADLYFLSALR-----WAAYT--PGRD-LAEELEKNLRLLLEGMLV-

General information: TITO was launched using: RESULT: Template: 3ANP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 692 -49380 -71.36 -266.92 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -71.36 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_3ANP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANP-query.scw PDB file : Tito_Scwrl_3ANP.pdb: