Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLPQQYRAKIIGYPSFGDTPESLEVKSFEDLTNYVVNQI----HDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLE--PFNVQ----DWR-----------------------EAYRQAFL---KYP-DWFIT--------TNANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 5EGN Chain:F ((20-261)) VPIVFLHPFSTNGGIWYFQTFPFAQTNHVIVIDHRGHGRSDKPATGYSIMEHADDVVAVLDALKVDRAVFVGNSIGGMIAMQLNLDHPQRVIGNLILSSGTGLGEGMPPEAGAAFQNDYIGAFGGLLEGAVSARSKRERPEILAVMKAHFSVPSNFPKHVFDAATADPNGVFAWNIKDRLSSIQAPTLVVAGEEDLVTTVANNQLLADNIPGAELRVINDVGHFYQLERPSEFNELLRGFVA---

General information: TITO was launched using: RESULT: Template: 5EGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 936 -39213 -41.89 -199.05 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain F : 0.69 3D Compatibility (PKB) : -41.89 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_5EGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGN-query.scw PDB file : Tito_Scwrl_5EGN.pdb: