Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEIKRIQQQPDLAQDIYAVMAAVYPVSPWTLEQIQADLSQDQTWYALAYDGAEVIGFLTVQETL------------FEAEVLQIAVKGAYQGQGIASALFA-----Q-L-PTDKEIFLEVRQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG 4R3L Chain:A ((12-167)) -FTLRNA--RMDDIDQIIKINRLTLPE-NYPYYFFVEHLKEYGLAFFVAIVDNSVVGYIMPRIEWGFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNFKKVKVLKGYYADG-EDAYLMARPL---

General information: TITO was launched using: RESULT: Template: 4R3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 610 -40437 -66.29 -295.16 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -66.29 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_4R3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R3L-query.scw PDB file : Tito_Scwrl_4R3L.pdb: