Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFSQANVMSRGIIGDLQGERVVASPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAVPQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMVPDRYEVTVIGEEPWGNYNRIMLSPVLSGEKTIEDIML-HPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKG-QTVDYDRLILATGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCG--SKNNAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMERQLDGRASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKDGTVLDADLVVFAVGIRPNIALAQSAGLRC--NRGVLVNDTMQTFDPSIYAVGECIEHRGQT------FGLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYE---D--IILN--------DEKRQIYKRIIIQ--NDRVIGAVLFGDTED--GMWYAELIADQTPVSSFRNKLLFGRDFALKNAG 4FX9 Chain:A ((5-420)) -------------------------------------------------------------------------------------------------------------------------------------MKKKVVIIGGGAAGMSAASRVKRLKP-EWDVKVFEATEWVSHAPCGIPYVVEGLSTPDKLMYYPPEVFIKKRGIDLHLNAEVIEVDTGYVRVR--GGEKSYEWDYLVFANGASPQVPAIEGVNLKGVFTADLPPDALAIREYMEKYKVENVVIIGGGYIGIEMAEAFAAQGKNVTMIVRGERVLRRSFDKEVTDILEEKLKK-HVNLRLQEITMKIEGEE--RV-EKVVTDAGEYKAELVILATGIKPNIELAKQLGVRIGETGAIWTNEKMQTSVENVYAAGDVAETRHVITGRRVWVPLAPAGNKMGYVAGSNIAGK-ELHFPGVLGTAVTKFMDVEIGKTGLTEMEALKEGYDVRTAFIKASTRPHYYPGGREIWLKGVVDNETNRLLGVQVVGSDILPRIDTAAAMLMAGFTTKDAFF--------------

General information: TITO was launched using: RESULT: Template: 4FX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2203 -73175 -33.22 -190.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -33.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_4FX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FX9-query.scw PDB file : Tito_Scwrl_4FX9.pdb: