Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNVSMTQRISEVVRNTNETKIRVRLNLDGTGQGTLNTGVPFLDHMIDQIKQHGLFDIDIHCDGDLEIDDHHTVEDCGITLGQAFAQALGDKKGLRRYGHFYAPLDEALSRVVVDLSGRPGLFMDIPFTRARIGTFDVDLFSEFFQGFVNHALMTLHIDNLKGKNSHHQIESVFKALARALRMACEIDPRAENTIASTKGSL 2AE8 Chain:C ((30-208)) --------MIYQKQR----TQLNISISDDQSPS-HINTGVGFLNHMLTLFTFHSGLSLNIEAQG----DDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLARVVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHEIEAIFKAFSRALGIALTAT--------------

General information: TITO was launched using: RESULT: Template: 2AE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 924 -145511 -157.48 -850.94 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -157.48 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_2AE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AE8-query.scw PDB file : Tito_Scwrl_2AE8.pdb: