Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDGLGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRFTLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDTIVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGLRGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREYLLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFEAAREMLIQTLKGLDKSTVNTIVYGK 2CF4 Chain:A ((3-332)) ---SMIEKLKKFTQIPGISGYEERIREEIIREIKDFA-DYKVDAIGNLIVELGE---GEERILFMAHMDEIGLLITGITDEGKLRFRKVGGIDDRLLYGRHVNVVTEKG-ILDGVIGATPPHLSLER-DKSVIPWYDLVIDIGAESKEEALEL-VKPLDFAVFKKHFSVLN-GKYVSTRGLDDRFGVVALIEAIKDLVDHELEGKVIFAFTVQEEVGLKGAKFLANHYYPQYAFAIDSFACCSPLTG---DVKLGKGPVIRAVDNSAIYSRDLARKVWSIAEKNGIEIQIGVTGGGTDASAFQ--D-RSKTLALSVPIKYLHSEVETLHLNDLEKLVKLIEALAFEL------------

General information: TITO was launched using: RESULT: Template: 2CF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1983 -201563 -101.65 -610.80 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -101.65 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_2CF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CF4-query.scw PDB file : Tito_Scwrl_2CF4.pdb: