Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADLHTSVFGIELDTPIIQAPSAAQGLAHE-KGEADTAKGVAAAGSIFS--ISTYANTTIKDAADAAPGAPQ--FFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFPLPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSPEDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKR-----VPVIFDSGVRRGEHVFKALASGADLVAIGRPVIYGLNLVGQKE 1KBI Chain:B ((140-444)) ----AWAYYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNT---------KTNVEESQGASRALSKFIDPS---LTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVLSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGR--

General information: TITO was launched using: RESULT: Template: 1KBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1472 51654 35.09 179.98 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : 35.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1KBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KBI-query.scw PDB file : Tito_Scwrl_1KBI.pdb: